Evaluation of protein separations based on hydrophobic interaction chromatography using polyethylene terephthalate capillary-channeled polymer (C-CP) fiber phases

Capillary-channeled Polymer Fibers as Stationary Phases for Protein Chromatography

The affinity ligand quality of the triazine dye, cibacron blue, toward serum albumin has been well studied [64-68]. By immobilizing the ligand to a stationary phase, affinity separations can be performed in order to remove bulk albumin from serum samples. This decrease in albumin concentration may serve as a direct advantage for proteomics as large amounts of serum albumin hinder the study of l...

Evaluation of synthesized lipid tethered ligands for surface functionalization of polypropylene capillary-channeled polymer fiber stationary phases.

Polypropylene (PP) capillary-channeled polymer (C-CP) fibers have been used in this laboratory as stationary phases for high performance liquid chromatography and solid phase extraction of proteins. Greater selectivity has been realized through the functionalization of the PP fibers through the physical adsorption of commercially available head group-modified poly(ethylene glycol) lipids (PEG-l...

Polymer-coated fibrous stationary phases in packed-capillary gas chromatography.

Fibrous synthetic polymers have been introduced as the support material for packed capillaries in gas chromatography. The filaments of the polymers were packed longitudinally into a fused-silica capillary, followed by the conventional coating process for open-tubular capillaries. With various polysiloxane-based polymeric materials coated onto these filaments, it was demonstrated that the retent...

Hydrophobic Interaction Chromatography

Hydrophobic Interaction Chromatography

modeling for the design of a Biomimetic chimeric ligand. Application to the puriRcation of bovine heart L-lactate dehydrogenase. Biotechnology and Bioengineering 63: 321}331. Lowe CR (1984) Applications of reactive dyes in biotechnology. In: Wiseman A (ed.) Topics in Enzyme and Fermentation Biotechnology, vol. 9. Chichester: Ellis Horwood. Lowe CR, Burton S, Pearson J, Clonis YD and Stead CV (1...